ADVANCES IN GENE KNOCKOUT TECHNOLOGY

Home Page

• Text​

  • (2.1)​ https://en.wikipedia.org/wiki/Gene_knockout

  • (2.2) http://www.discoverymedicine.com/Benjamin-Yang/2009/05/10/three-scientists-who-invented-gene-knockout-technology-won-lasker-basic-medical-research-award/

  • (2.3) https://www.reuters.com/article/us-health-embryos-editing/scientists-edit-embryos-genes-to-study-early-human-development-idUSKCN1BV2F0

  • (2.4) https://www.23andme.com/gen101/genes/

  • (2.5) http://www.sciencemag.org/news/2017/04/human-knockouts-may-reveal-why-some-drugs-fail

  • (2.6) https://www.sciencedaily.com/releases/2017/12/171219133617.htm

  • (2.7)​ https://www.sciencedaily.com/releases/2015/04/150430091829.htm

  • (2.8) http://learn.genetics.utah.edu/content/genetherapy/challenges/

  • (2.9) https://www.reuters.com/article/us-health-embryos-editing/scientists-edit-embryos-genes-to-study-early-human-development-idUSKCN1BV2F0

  • (2.10) https://www.ncbi.nlm.nih.gov/books/NBK21632/

  • (2.11) https://www.nature.com/articles/nbt.3931

  • (2.12) https://www.sciencedaily.com/releases/2018/03/180308143052.htm

  • (2.13) http://www.noldus.com/blog/zebrafish-down-syndrome-gene-autism

  • (2.14) http://www.newsweek.com/alzheimers-disease-completely-reversed-removing-just-one-enzyme-new-study-807156

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• Images/Videos

  • (P2.1)​ https://www.123rf.com/photo_33833798_hand-of-a-lab-technician-holding-blood-tube-test-and-background-a-rack-of-color-tubes-with-blood-sam.html

  • (P2.2) https://encrypted-tbn0.gstatic.com/images?q=tbn:ANd9GcRcdMiAsaIvzmZ89chpId4XF08NYIL-Lb_ayOEZ3oZr4F_BfCyYGg

  • (P2.3) https://sherwoodwellness.tv/genes-affect-obesity/

  • (P2.4) https://www.youtube.com/watch?v=5MQdXjRPHmQ

  • (P2.5) https://ghr.nlm.nih.gov/primer/illustrations/autodominant.jpg

  • (P2.6) http://images.fineartamerica.com/images-medium-large/12-chromosomes-artwork-sciepro.jpg

  • (P2.7) https://media.nature.com/m685/nature-assets/nbt/journal/v35/n9/images/nbt.3931-F1.jpg

  • (P2.8) https://www.psychologicalscience.org/redesign/wp-content/uploads/2016/01/PAFF_082917_flippingbook-609x419.jpg

  • (P2.9) https://cdn1.medicalnewstoday.com/content/images/articles/150/150670/malaria-is-passed-on-by-the-anopheles-mosquito.jpg

  • (P2.10) https://ichemepresident.files.wordpress.com/2014/10/zebrafish.jpg

  • (P2.11) http://cdn.natgeotv.com.au/factsheets/thumbnails/MousePlagueHeader.jpg?v=28&azure=false&scale=both&width=1024&height=560&mode=crop

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Methods

• Text​

  • (5.1) https://www.cellecta.com/technology-portfolio/crispr-cas9-for-gene-knockout/

  • (5.2) https://redrecombineering.ncifcrf.gov/protocols/step-by-step-knockout-dsdna.pdf

  • (5.3) https://en.wikipedia.org/wiki/Transposon_mutagenesis#Practical_applications

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• Images/Videos

  • (P5.1)​ https://www.cellecta.com/wp-content/uploads/CRISPR_diagram_Genomic_DNA.jpg

  • (P5.2) http://www.youtube.com/watch?v=MzMsgmeEOhI

  • (P5.3) https://www.iteratemarketing.com/wp-content/uploads/2016/10/header-pipettes-580x366.jpg

  • (P5.4) https://upload.wikimedia.org/wikipedia/commons/thumb/4/4b/Sleeping_Beauty_transposon.jpg/400px-Sleeping_Beauty_transposon.jpg

  • (P5.5) http://www.genecopoeia.com/wp-content/uploads/2014/08/Pathways-for-repair-of-DSBs.jpg

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History

• Text

  • (1.1) https://www.dna-worldwide.com/resource/160/history-dna-timeline

  • (1.2) https://unlockinglifescode.org/timeline

  • (1.3) http://www.bbc.co.uk/schools/gcsebitesize/science/add_edexcel/cells/dnarev3.shtml

  • (1.4) https://hms.harvard.edu/news/harvard-medicine/expanded-timeline-personalized-medicine

  • (1.5) https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/gene-knockout

  • (1.6) https://www.sciencedaily.com/releases/2018/02/180202112624.htm

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• Images/Videos

  • (P1.1)​ https://upload.wikimedia.org/wikipedia/commons/b/ba/Gregor_Mendel_2.jpg

  • (P1.2) https://upload.wikimedia.org/wikipedia/en/thumb/9/97/Rosalind_Franklin.jpg/220px-Rosalind_Franklin.jpg

  • (P1.3) http://www.nobelprize.org/nobel_prizes/medicine/laureates/1962/wilkins.jpg

  • (P1.4) https://upload.wikimedia.org/wikipedia/commons/thumb/1/10/Marshall_Nirenberg_performing_experiment.jpg/1200px-Marshall_Nirenberg_performing_experiment.jpg

  • (P1.5) https://unlockinglifescode.org/sites/default/files/1961.jpg

  • (P1.6) http://www.achievement.org/wp-content/uploads/2017/04/GettyImages-515174458_master.jpg

  • (P1.7)​ https://labs.uab.edu/mccgray/images/HD_brains.jpg

  • (P1.8) https://upload.wikimedia.org/wikipedia/commons/e/e1/Protein_BRCA1_PDB_1jm7.png

  • (P1.9) https://unlockinglifescode.org/sites/default/files/2003.jpg

  • (P1.10) https://media-exp2.licdn.com/mpr/mpr/AAEAAQAAAAAAAAQaAAAAJDAxZWM0ZGNmLWM2NzMtNDk2OS1iNTNhLThhNzYwNzI3ZWQ4Ng.jpg

  • (P1.11) http://genome.wustl.edu/image/images/misc/circularcancergenfree.jpg

  • (P1.12) http://www.sabiosciences.com/images/CNV_Fig1_480.jpg

  • (P1.13) http://www.laskerfoundation.org/media/filer_public_thumbnails/filer_public/b3/10/b310de56-87cc-4915-91eb-b4828b729b78/capecchi_mario.jpg__350x350_q85_crop_subsampling-2_upscale.jpg

  • (P1.14) http://www.sciencemag.org/sites/default/files/styles/article_main_image_-_1280w__no_aspect_/public/crispr_mice_1280.jpg?itok=DnitRSra

  • (P1.15) https://www.ospe.on.ca/policy-areas/research-innovation

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Applications

• Text

  • (6.1)​ 

  • (6.2) http://www.sciencemag.org/news/2017/04/human-knockouts-may-reveal-why-some-drugs-fail

  • (6.3) https://www.eurekalert.org/pub_releases/2017-04/uops-gof041217.php

  • (6.4) https://www.sciencenews.org/article/gene-knockouts-people-provide-drug-safety-effectiveness-clues

  • (6.5) http://www.philly.com/philly/health/Penn-Pakistan-triglycerides-knockouts-genetics-amish-merck.html

  • (6.6) http://sitn.hms.harvard.edu/flash/2015/xenotransplantation-can-pigs-save-human-lives/

  • (6.7)​ http://www.pnas.org/content/101/47/16501

  • (6.8) https://www.researchgate.net/profile/Prakash_Singh6/publication/264897740_Gene_Knock_Out_Technology_and_Its_Application_In_Singh_N_and_Kumar_A_Eds_BIOLOGIX_Vol_II/links/53f4f11f0cf2888a7491369c/Gene-Knock-Out-Technology-and-Its-Application-In-Singh-N-and-Kumar-A-Eds-BIOLOGIX-Vol-II.pdf

  • (6.9) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5617878/

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• Images/Videos

  • (P6.1)​ https://assets.nerdwallet.com/blog/wp-content/uploads/2016/05/difference-between-generic-brand-name-drugs.jpg

  • (P6.2) https://www.newsmax.com/CMSPages/GetFile.aspx?guid=99295c45-9c46-40b5-a3db-fc51a28b52d7&SiteName=Newsmax

  • (P6.3) http://www.diversionspecialists.com/wp-content/uploads/magnifying-glass-capsules-syringes-shutterstock_188508998-copy-598x364.jpeg

  • (P6.4) https://litronlabs.com/getmetafile/54df6665-9811-4308-ba1c-2ceb23b045bd/In-Vivo-Pig-a-copy.aspx?maxsidesize=400)

  • (P6.5) http://www.philly.com/philly/health/Penn-Pakistan-triglycerides-knockouts-genetics-amish-merck.html

  • (P6.6) http://images.medicaldaily.com/sites/medicaldaily.com/files/styles/headline/public/2014/10/29/sperm-health.jpg

  • (P6.7)​ https://www.naturopathiccurrents.com/images/v25%20june%202015/male-infertility-1.jpg

  • (P6.8) https://i0.wp.com/sitn.hms.harvard.edu/wp-content/uploads/2015/11/Xenotransplantation3.jpg?resize=1024%2C713

  • (P6.9) https://media.istockphoto.com/photos/sheep-herd-in-new-zealand-picture-id155379351?k=6&m=155379351&s=612x612&w=0&h=iu7EfpVCY_OOjs1xRZcwfnejWOGh9z6LINs2_v7hYhk=

  • (P6.10) https://www.corning.com/cala/en/products/communication-networks/applications.html

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Innovators

• Text

  • (3.1) https://en.wikipedia.org/wiki/Mario_Capecchi#Knockout_mice

  • (3.2) https://www.genome.gov/17515708/

  • (3.3) https://www.ucdavis.edu/news/mouse-genome-studies-show-disease-models-and-sex-differences

  • (3.4) https://www.sciencedaily.com/releases/2015/04/150430091829.htm

  • (3.5) https://innovativegenomics.org/blog/improved-knockout-cas9/

  • (3.6) https://en.wikipedia.org/wiki/National_Institutes_of_Health

  • (3.7)​ https://www.reuters.com/article/us-health-embryos-editing/scientists-edit-embryos-genes-to-study-early-human-development-idUSKCN1BV2F0

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• Images/Videos

  • (P3.1)​ http://neuroscience.med.utah.edu/_images/faculty/Capecchi.jpg

  • (P3.2) https://healthcare.utah.edu/capecchi/images/lab.jpg

  • (P3.3) https://media.eurekalert.org/multimedia_prod/pub/web/125546_web.jpg

  • (P3.4) http://cuny2018.ucdavis.edu/uploads/5/3/8/1/53814405/welcome-sign_orig.jpg

  • (P3.5) https://www.nature.com/polopoly_fs/7.26843.1433771123!/image/Mouse3.jpg_gen/derivatives/landscape_630/Mouse3.jpg

  • (P3.6) https://www.nature.com/naturejobs/science/system/logos/000/140/891/resized/uth_logo.png

  • (P3.7)​ https://www.sciencenewsforstudents.org/sites/default/files/scald-image/860_main_knockout_gene.png

  • (P3.8) https://www.autismspeaks.org/sites/default/files/images/features/nih.logo_.feature.jpg?1452715932

  • (P3.9) https://www.gizmodo.com.au/2017/08/its-official-for-the-first-time-ever-us-scientists-have-edited-the-dna-of-a-human-embryo/

  • (P3.10) http://complexspinesurgery.com//wp-content/uploads/2016/02/innovator_header102717.jpg

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Issues

• Text

  • (4.1) https://ghr.nlm.nih.gov/primer/therapy/ethics

  • (4.2) https://www.ncbi.nlm.nih.gov/books/NBK19931/

  • (4.3) https://www.omicsonline.org/open-access/ethical-issues-in-genome-editing-using-crisprcas9-system-2155-9627-1000266.php?aid=70914

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• Images/Videos

  • (P4.1) http://ak7.picdn.net/shutterstock/videos/4781507/thumb/1.jpg

  • (P4.2) http://cdn.skilledup.com/wp-content/uploads/2014/11/20233-Law-1290x688-940x501.jpg

  • (P4.3) http://longevityfacts.com/rejuvenate-bodies-hgh-boosting-gene-therapy-growth-hormone-releasing-hormone-ghrh-plasmid-therapy/​